Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed
View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

DyLight 649 is excited at 646 (in PBS) and emits at 674 (in PBS).

Scientific Data Images

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) [NBP1-51898] - Analysis of c-Myc in human osteosarcoma cells using goat anti-mouse DyLight 649 conjugated secondary antiboy. Image from verified customer review.
Immunohistochemistry-Frozen: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) [NBP1-51898] - Analysis of mouse tissue using goat anti-mouse DyLight 649 conjugated secondary antibody. Image from verified customer review.
Flow Cytometry: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) [NBP1-51898] - Analysis of human osteosarcoma cells using goat anti-mouse DyLight 649 conjugated secondary antibody. Image from verified customer review.

Applications

Application
Recommended Usage

Flow Cytometry

1:50 - 1:200

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:50 - 1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:50 - 1:500

Reviewed Applications

Read 4 reviews rated 4 using NBP1-51898 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS containing 0.2% BSA

Format

Pre-adsorbed

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews (4)

4 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Name: Arun Satelli
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse tissue
    Species: Mouse
    Verified Customer | Posted 10/23/2014
    Dylight 649 IHC frozen
    Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) NBP1-51898
  • Name: Arun Satelli
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human pancreatic tissue
    Species: Human
    Verified Customer | Posted 10/23/2014
    Dylight 649 IHC paraffin
    Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) NBP1-51898
  • Name: Arun Satelli
    Application: Immunocytochemistry
    Sample Tested: Human osteosarcoma cell line
    Species: Human
    Verified Customer | Posted 10/23/2014
    Dylight 649 immunocytochemistry
    Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) NBP1-51898
  • Name: Arun Satelli
    Application: Flow Cytometry
    Sample Tested: Human osteosarcoma cell line
    Species: Human
    Verified Customer | Posted 10/23/2014
    Dylight 649 Flow
    Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) NBP1-51898

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Showing  1 - 4 of 4 reviews Showing All

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