Key Product Details

Species Reactivity

Canine

Applications

Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Goat IgG
View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Dog IgG F(c) fragment

Specificity

No reaction was observed against Dog IgG F(ab).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Dog IgG, Dog IgG F(c) and Dog Serum

Applications for Goat anti-Canine IgG Fc Secondary Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

1:500 - 1:2500

Fluorophore-linked immunosorbent assay

1:10000 - 1:50000

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Goat anti-Canine IgG Fc Secondary Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Goat anti-Canine IgG Fc Secondary Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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