Sheep anti-Canine IgG Fc Secondary Antibody

Name: Sheep anti-Canine IgG Fc Secondary Antibody
Brand: Novus Biologicals
SKU: NBP2-69475
Price: 269 USD
Availability: InStock
Rating: 4.5 (2 reviews)

Novus Biologicals | Catalog # NBP2-69475

(2)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Canine

Applications

Immunoelectrophoresis, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Sheep

View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Highly purified normal IgG isolated from pooled dog serum was used for immunization of the sheep. Freund's complete adjuvant is used in the first step of the immunization procedure.

Reactivity Notes

The antiserum does not cross-react with any other component of the Dog immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Specificity

The reactivity of the antiserum is restricted to the Fc part of the IgG molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal dog serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments.

Clonality

Polyclonal

Host

Sheep

Description

The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.

Scientific Data Images for Sheep anti-Canine IgG Fc Secondary Antibody

ELISA: Sheep anti-Canine IgG Fc Secondary Antibody [NBP2-69475]

ELISA: Sheep anti-Canine IgG Fc Secondary Antibody (Azide Free) [NBP2-69475] - ELISA image submitted by a verified customer review.

Applications for Sheep anti-Canine IgG Fc Secondary Antibody

Application

Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Application Notes

Precipitation assays. In immunoelectrophoresis use 2 ul serum or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells..

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-69475 in the following applications:

ELISA (2 Reviews)

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Reconstitution

Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Formulation

Whole antiserum

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Product Documents for Sheep anti-Canine IgG Fc Secondary Antibody

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Certificate of Analysis

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Product Specific Notices for Sheep anti-Canine IgG Fc Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Sheep anti-Canine IgG Fc Secondary Antibody (2)

4.5 out of 5

2 Customer Ratings

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Sheep anti-Canine IgG Fc fragment Secondary Antibody (Azide Free)

Name: Anonymous

Application: ELISA

Sample Tested: EDTA Plasma

Species: Rabbit

Verified Customer | Posted 12/06/2020

one
Sheep anti-Canine IgG Fc Secondary Antibody (Azide Free)

Name: Anonymous

Application: ELISA

Sample Tested: k2edta plasma

Species: cynomolgus

Verified Customer | Posted 07/06/2020

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