Bovine IgG Fc Isotype Control

Novus Biologicals | Catalog # NBP1-97044

Novus Biologicals
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Key Product Details

Species Reactivity

Bovine

Applications

Western Blot, ELISA, Dot Blot, SDS-Page

Label

Unconjugated

Antibody Source

Bovine IgG

Format

Purified
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Product Specifications

Specificity

No reaction was observed against anti-Bovine IgG F(ab')2 or anti-Papain.

Host

Bovine

Isotype

IgG

Description

This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(c)

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Bovine IgG Fc Isotype Control

Dot Blot: Bovine IgG Fc Isotype Control [NBP1-97044] - Dot Blot of Bovine IgG FcFragment.Lane 1: 100 ng Bovine Fc.Lanes 2-5: serial dilution 3 fold of Antigen.Primary Antibody: Rabbit Anti-Bovine Fc at 1 ug/ml at RT for 1 hour.Secondary Antibody: Rabbit Secondary HRP Antibody at 1:70,000 for 30 min at RT.Block at RT for 30 minutes.
SDS-PAGE: Bovine IgG Fc Isotype Control [NBP1-97044]

SDS-PAGE: Bovine IgG Fc Isotype Control [NBP1-97044]

SDS-Page: Bovine IgG Fc Isotype Control [NBP1-97044] - SDS-PAGE for Bovine IgG Fc isotype control.Lane 1: 1ug Reduced Bovine Fc.Lane 2: MW ladder Opal pre-stained.Lane 3: 1ug Non-Reduced Bovine Fc.Coomassie Blue Stained.
Bovine IgG Fc Isotype Control

Bovine IgG Fc Isotype Control

Dot Blot of Bovine IgG Fc Isotype Control. Lane 1: 100 ng Bovine Fc. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: Rabbit Anti-Bovine Fc

Applications for Bovine IgG Fc Isotype Control

Application
Recommended Usage

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

SDS-Page

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product has been tested in SDS-Page and Dot Blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Purified

Preservative

0.01% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.

Product Documents for Bovine IgG Fc Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Bovine IgG Fc Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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FAQs

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