Key Product Details

Species Reactivity

Goat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Polyclonal Donkey IgG

Format

Pre-adsorbed
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Product Specifications

Immunogen

Goat IgG whole molecule

Specificity

This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Description

This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum

Scientific Data Images for Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7590] - Western blot of Donkey anti-Goat IgG (H+L) Secondary antibody [PE] (Pre-adsorbed).Lane 1: Goat IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Donkey anti-Goat IgG (H+L) Secondary antibody [PE] (Pre-adsorbed) at 1:1,000 for 60 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG.Other band(s): None.
Dot Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) [NB7590]
Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Dot Blot of Donkey anti-Goat IgG Antibody Phycoerythrin Conjugated. Antigen: Goat IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: none. Secondary antibody: Donkey anti-Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)at 1:1,000 for 60 min at RT. Block for 1 HR at RT.

Applications for Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Application
Recommended Usage

Flow Cytometry

1:100 - 1:250

Immunocytochemistry/ Immunofluorescence

1:100 - 1:250
Application Notes
This product has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Specific conditions for reactivity should be optimized by the end user.

Reviewed Applications

Read 2 reviews rated 4 using NB7590 in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Format

Pre-adsorbed

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: IgG (H+L)

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Additional IgG (H+L) Products

Product Documents for Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Customer Reviews for Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) (2)

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human cancer cell line
    Species: Human
    Verified Customer | Posted 11/25/2014
    Human cancer cell line
    Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) NB7590
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human osteosarcoma cell line
    Species: Human
    Verified Customer | Posted 11/25/2014
    Human osteosarcoma cell line
    Donkey anti-Goat IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) NB7590

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