IgG1 Antibody (RM106) [Biotin]

Novus Biologicals | Catalog # NBP2-62006B

Recombinant Monoclonal Antibody.
Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM106 expressed in HEK293
View all IgG1 Primary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

This antibody reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mouse IgG2b, and may also react to goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG1 Antibody (RM106) [Biotin]

Western Blot: IgG1 Antibody (RM106) [Biotin] [NBP2-62006B] - Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20 ng/lane), using 0.2 ug/mL NBP2-62006B. This antibody reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced form (~50 kDa).
IgG1 Antibody (RM106) [Biotin]

ELISA: IgG1 Antibody (RM106) [Biotin] [NBP2-62006B] -

ELISA: IgG1 Antibody (RM106) [Biotin] [NBP2-62006B] - A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of NBP2-62006B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG1 Antibody (RM106) [Biotin]

ELISA: IgG1 Antibody (RM106) [Biotin] [NBP2-62006B] -

ELISA: IgG1 Antibody (RM106) [Biotin] [NBP2-62006B] - ELISA of mouse immunoglobulins shows NBP2-62006B reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with different immunoglobulins.NBP2-62006B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG1 Antibody (RM106) [Biotin]

Application
Recommended Usage

ELISA

0.005 ug/ml-0.2 ug/ml

Western Blot

0.1 ug/ml - 0.5 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG1

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for IgG1 Antibody (RM106) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IgG1 Antibody (RM106) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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