Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed
View all IgG2A Secondary Antibodies »

Product Specifications

Immunogen

IgG2a paraproteins from BALB/c mice

Specificity

Goat anti Mouse IgG2a antibody recognizes Mouse IgG2a. This antibody has been pre-absorbed against mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. The antibody shows minimal cross-reactivity with human immunoglobulins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-Mouse IgG2a Secondary Antibody [FITC] (Pre-adsorbed)

Goat anti-Mouse IgG2a Secondary Antibody [FITC] (Pre-adsorbed) Flow Cytometry: Goat anti-Mouse IgG2a Secondary Antibody [FITC] - (Pre-adsorbed) [NBP2-68509] -

Flow Cytometry: Goat anti-Mouse IgG2a Secondary Antibody [FITC] - (Pre-adsorbed) [NBP2-68509] -

Flow Cytometry: Goat anti-Mouse IgG2a Secondary Antibody [FITC] - (Pre-adsorbed) [NBP2-68509] - Figure A. Purified mouse anti porcine CD6 detected with goat anti mouse IgG2a PE and mouse IgG2b FITC isotype control. Figure B. Purified mouse anti porcine CD6 detected with goat anti mouse IgG2a PE and mouse anti porcine CD45 FITC. All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Applications for Goat anti-Mouse IgG2a Secondary Antibody [FITC] (Pre-adsorbed)

Application
Recommended Usage

Flow Cytometry

1:1000

Immunofluorescence

Neat - 1:10

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

Pre-adsorbed

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Goat anti-Mouse IgG2a Secondary Antibody [FITC] (Pre-adsorbed)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Mouse IgG2a Secondary Antibody [FITC] (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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