Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MPC-11
View all IgG2B Isotype Controls »

Product Specifications

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This Mouse IgG2B (MPC-11) immunoglobulin is useful as an isotype control for Mouse IgG2B (MPC-11) antibody.

Scientific Data Images for Mouse IgG2b Isotype Control (MPC-11)

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [FITC] [NBP2-27229] - TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left), or TLR4-FITC (right). PPI negative, CD14+ cells were gated for analysis. Image using the FITC form of this antibody.
Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Analysis of Alexa Fluor (R) 488 conjugate of NBP2-27231. Cell surface 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control. FITC-conjugated goat anti-mouse secondary
Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Cell surface analysis of 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control. FITC-conjugated goat anti-mouse secondary antibody was used for this experiment.
Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.
Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 488] [NBP2-27231AF488] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.
Flow (Cell Surface): Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow (Cell Surface): Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor (R) 647] [NBP2-27228] - Cell surface analysis of CD86 in human monocytes using 0.1 ug of NBP2-00308 conjugated to Alexa Fluor 647. The shaded histogram represents human monocytes alone; green represents mouse isotype control ; red represents CD86 this antibody. Image using the Alexa Fluor 647 form of this antibody.
Flow (Cell Surface): Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow (Cell Surface): Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 700] [NBP2-27231AF700] - A surface stain was performed on hPBMCs with CCR1 (53504) antibody FAB145N and a matched isotype control NBP2-27231AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image using the Alexa Fluor 700 form of this antibody.
Flow (Cell Surface): Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow (Cell Surface): Mouse IgG2b Isotype Control (MPC-11) [NBP2-27231]

Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Allophycocyanin] [NBP2-27231APC] - A surface stain was performed on human peripheral blood monocytes with EMMPRIN/CD147 (MEM-M6/2) antibody NB500-561APC (blue) and a matched isotype control NBP2-27231APC (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.

Applications for Mouse IgG2b Isotype Control (MPC-11)

Application
Recommended Usage

Flow Cytometry

1:20 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500
Application Notes
Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG2B

A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens. IMGENEX offers a variety of mouse isotype controls including fluorescene conjugates and azide-free formats.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Mouse IgG2b Isotype Control (MPC-11)

Certificate of Analysis

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Product Specific Notices for Mouse IgG2b Isotype Control (MPC-11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Mouse IgG2b Isotype Control (MPC-11)

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Protocols

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