Mouse IgG2b Kappa Isotype Control (M2BK)
Novus Biologicals | Catalog # NBP1-96980
![Flow Cytometry: Mouse IgG2b Kappa Isotype Control (M2BK) [NBP1-96980]](https://resources.rndsystems.com/images/products/Mouse-IgG2b-Kappa-Isotype-Control-M2BK-NBP1-96980-img0001.jpg "Flow Cytometry: Mouse IgG2b Kappa Isotype Control (M2BK) [NBP1-96980]")
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Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # M2BK
Format
Purified
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Product Specifications
Specificity
Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Mouse IgG2b Kappa Isotype Control (M2BK)
Flow Cytometry: Mouse IgG2b Kappa Isotype Control (M2BK) [NBP1-96980]
Flow Cytometry: Mouse IgG2b Kappa Isotype Control (M2BK) [NBP1-96980] - Cells were stained with the positive antibody (right) and Isotype control (left) and unstained cells for 1h at 4C. Image from verified customer review.Applications for Mouse IgG2b Kappa Isotype Control (M2BK)
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:1000 - 1:5000
Fluorophore-linked immunosorbent assay
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Product Documents for Mouse IgG2b Kappa Isotype Control (M2BK)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Mouse IgG2b Kappa Isotype Control (M2BK)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
Customer Reviews for Mouse IgG2b Kappa Isotype Control (M2BK) (2)
5 out of 5
2 Customer Ratings
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Verified Customer | Posted 02/27/2019Cells were stained with the positive antibody (right) and Isotype control (left) and unstained cells for 1h at 4C
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Verified Customer | Posted 06/28/2018antibody staining negative with isotype control
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