IgG4 Fc Antibody (RM217) [Biotin]

Novus Biologicals | Catalog # NBP2-62127B

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Flow Cytometry

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM217 expressed in HEK293
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Product Specifications

Immunogen

Peptide corresponding to the Fc region of Human IgG4

Specificity

This antibody reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG4 Fc Antibody (RM217) [Biotin]

IgG4 Fc Antibody (RM217) [Biotin]

ELISA: IgG4 Fc Antibody (RM217) [Biotin] [NBP2-62127B] -

ELISA: IgG4 Fc Antibody (RM217) [Biotin] [NBP2-62127B] - Sandwich ELISA using NBP2-62127B as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.
IgG4 Fc Antibody (RM217) [Biotin]

Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] -

Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.
IgG4 Fc Antibody (RM217) [Biotin]

ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] -

ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] - A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG4 Fc Antibody (RM217) [Biotin]

Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] -

Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP2-62127B] - RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3

Applications for IgG4 Fc Antibody (RM217) [Biotin]

Application
Recommended Usage

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Product Documents for IgG4 Fc Antibody (RM217) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IgG4 Fc Antibody (RM217) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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