IgG4 Fc Antibody (RM217) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25978
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM217 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Peptide corresponding to the Fc region of Human IgG4
Specificity
This antibody reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IgG4 Fc Antibody (RM217) - Azide and BSA Free
Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] -
Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] -
Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3ELISA: IgG4 Fc Antibody (RM217) [NBP3-25978] -
ELISA: IgG4 Fc Antibody (RM217) [NBP3-25978] - Sandwich ELISA using biotinylated RM217 (NBP2-62127B) as the capture antibody, and biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] -
Multiplex Immunoassay: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] -
ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] -
Sandwich ELISA: IgG4 Fc Antibody (RM217) - Azide and BSA Free [NBP3-25978] - Sandwich ELISA using RM217 as the capture antibody, and NBP3-18532 as the detection antibody, followed by an AP conjugated streptavidin.Applications for IgG4 Fc Antibody (RM217) - Azide and BSA Free
Application
Recommended Usage
Sandwich ELISA Capture
50 - 200ng/well
Sandwich ELISA Detection
0.05 - 0.2ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Product Documents for IgG4 Fc Antibody (RM217) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IgG4 Fc Antibody (RM217) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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