IgM Antibody (SPM557)

Novus Biologicals | Catalog # NBP2-34375

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM557
View all IgM Primary Antibodies »

Product Specifications

Immunogen

Recombinant heavy chain of human IgM

Localization

Cytoplasm, Cell Surface and Secreted

Marker

B-Cell Marker

Specificity

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34422)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for IgM Antibody (SPM557)

Immunocytochemistry/ Immunofluorescence: IgM Antibody (SPM557) [NBP2-34375]

Immunocytochemistry/ Immunofluorescence: IgM Antibody (SPM557) [NBP2-34375]

Immunocytochemistry/Immunofluorescence: IgM Antibody (SPM557) [NBP2-34375] - Analysis using the DyLight 550 conjugate of NBP2-34375. Staining of LM7 cells using anti-IgM antibody [DyLight 550]. DAPI Solution (blue; cat.# NBP2-31156) was used as a nuclear counterstain. Image from verified customer review.
Immunohistochemistry-Paraffin: IgM Antibody (SPM557) [NBP2-34375]

Immunohistochemistry-Paraffin: IgM Antibody (SPM557) [NBP2-34375]

Immunohistochemistry-Paraffin: IgM Antibody (SPM557) [NBP2-34375] - Formalin-fixed, paraffin-embedded human Tonsil stained with igM Monoclonal Antibody (SPM557)

Applications for IgM Antibody (SPM557)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

UniProt

P01871 (Human), P20769 (Human)

Additional IgM Products

Product Documents for IgM Antibody (SPM557)

Certificate of Analysis

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Product Specific Notices for IgM Antibody (SPM557)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IgM Antibody (SPM557)

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Protocols

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