IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide and BSA Free

Name: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-33247
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-33247

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 39A1413

Format

Azide and BSA Free

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Product Specifications

Immunogen

This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa.

Modification

p Ser32, p Ser36

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide and BSA Free

Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free [NBP2-33247] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody. Image from the standard format of this antibody.

Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide Free [NBP2-33247] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nM TNF-a (lane 1). The membrane was blotted with NB100-56724 and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa. Image from the standard format of this antibody.

Applications for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide and BSA Free

Application

Recommended Usage

Immunoprecipitation

1-2 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide and BSA Free

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Product Specific Notices for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.