IKK epsilon/IKBKE Antibody (5N4J8)

Name: IKK epsilon/IKBKE Antibody (5N4J8)
Brand: Novus Biologicals
SKU: NBP3-16192
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16192

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5N4J8 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IKK epsilon/IKBKE (Q14164). MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLES

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKK epsilon/IKBKE Antibody (5N4J8)

Western Blot: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192]

Western Blot: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] - Western blot analysis of extracts of various cell lines, using IKK epsilon/IKBKE Rabbit mAb (NBP3-16192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry-Paraffin: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192]

Immunohistochemistry-Paraffin: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] - Immunohistochemistry of paraffin-embedded mouse heart using IKK epsilon/IKBKE Rabbit mAb (NBP3-16192) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] -

Immunohistochemistry: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] - Immunohistochemistry analysis of paraffin-embedded rat spleen tissue using IKK epsilon/IKBKE Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] -

Immunohistochemistry: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] - Immunohistochemistry analysis of paraffin-embedded rat colon tissue using IKK epsilon/IKBKE Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] -

Immunohistochemistry: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] - Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using IKK epsilon/IKBKE Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] -

Immunohistochemistry: IKK epsilon/IKBKE Antibody (5N4J8) [NBP3-16192] - Immunohistochemistry analysis of paraffin-embedded human colon tissue using IKK epsilon/IKBKE Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

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Applications for IKK epsilon/IKBKE Antibody (5N4J8)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IKK epsilon

Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.

Long Name

IkB Kinase epsilon

Alternate Names

IKBKE, IKK-I

Gene Symbol

IKBKE

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Product Specific Notices for IKK epsilon/IKBKE Antibody (5N4J8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.