Recombinant Bovine IL-1 alpha/IL-1F1 Protein

Name: Recombinant Bovine IL-1 alpha/IL-1F1 Protein
Brand: Novus Biologicals
SKU: NBP2-62430
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-62430

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

Pichia pastoris

Accession Number

P08831

Applications

ELISA, Functional Assay

View all IL-1 alpha/IL-1F1 Proteins and Enzymes »

Product Specifications

Description

Recombinant bovine interleukin-1 alpha.

Source:Pichia pastoris

Amino Acid Sequence:aa 120-268 of native bovine IL-1a, UniProt P08831

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Bovine IL-1 alpha/IL-1F1 Protein

ELISA: Recombinant Bovine IL-1 alpha/IL-1F1 Protein [NBP2-62430] -

ELISA: Recombinant Bovine IL-1 alpha/IL-1F1 Protein [NBP2-62430] - Bovine Interleukin-1 alpha ELISA

Recombinant Bovine interleukin-1 alpha detected using Rabbit anti Bovine interleukin-1 alpha as the capture reagent and biotinylated Rabbit anti Bovine interleukin-1 alpha as the detection reagent followed by Streptavidin:HRP.

Formulation, Preparation, and Storage

NBP2-62430

Preparation Method	Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Formulation	PBS
Preservative	10% Trehalose
Concentration	Lyoph
Reconstitution	Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-1 alpha/IL-1F1

Interleukin-1 alpha (IL-1a, lymphocyte activating factor (LAF), hematopoietin-1, endogeneous pyrogen, mononuclear cell factor) is a pleiotropic pro-inflammatory cytokine produced in a variety of cells, including monocytes, macrophages and epithelial cells. It is involved in inflammatory and immune responses and regulation of hematopoiesis. It may therefore be of clinical use following massive immunosuppression and chemotherapy. Recombinant human IL-1a is a single non-glycosylated polypetide chain of 159 amino acids with a MW of 18 kDa. Sequence conservation of the mature protein among various species is 60% -70%, human IL-1 has been found to be biologically active on murine cell lines.

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Gene Symbol

IL1A

UniProt

P08831 (Bovine)

Additional IL-1 alpha/IL-1F1 Products

Product Documents for Recombinant Bovine IL-1 alpha/IL-1F1 Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Bovine IL-1 alpha/IL-1F1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.