Human IL-1 RI ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-06802

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 uL)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human IL-1 RI in samples. No significant cross-reactivity or interference between Human IL-1 RI and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV 5.18

Inter-Assay Precision (Precision between assays) %CV 5.51

Recovery for Human IL-1 RI ELISA Kit (Colorimetric)

Recovery

87 - 107%

Linearity

Human IL-1 RI ELISA Kit (Colorimetric)

Scientific Data Images for Human IL-1 RI ELISA Kit (Colorimetric)

Human IL-1 RI ELISA Kit (Colorimetric)

ELISA: Human IL-1 RI ELISA Kit (Colorimetric) [NBP3-06802] -

ELISA: Human IL-1 RI ELISA Kit (Colorimetric) [NBP3-06802] - Standard Curve Reference

Kit Contents for Human IL-1 RI ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-1 RI

The Interleukin 1 receptor type I (IL-1R1) belongs to the IL-1 receptor family (1). IL-1R1 binds to IL-1 alpha, IL-1 beta, and interleukin-1 receptor antagonist protein (IL-1RA). IL-1RA regulates IL-1 ligand biological activity by a competitive interaction for IL-1R1. Upon IL-1 stimulation, adaptor molecule MyD88 is first recruited to the IL-1R1-IL1R accessory protein complex, followed by the recruitment of two serine-threonine kinases, IRAK4 and IRAK, and the adaptor protein TRAF6, resulting in activation of the NF-kB pathway (2-3). Stimulation of IL-1R1, leads to activation of the transcription factors NF-B, ATF, and AP-1.

Long Name

Interleukin 1 Receptor I

Alternate Names

CD121a, IL-1RI, IL1R1, IL1RI

Gene Symbol

IL1R1

Additional IL-1 RI Products

Product Documents for Human IL-1 RI ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human IL-1 RI ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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