IL-10R alpha Antibody (1B1.3a)

Novus Biologicals | Catalog # NBP3-07330

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Human (Negative)

Applications

Neutralization, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 kappa Clone # 1B1.3a
View all IL-10 R alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant extracellular region of Mouse IL-10R alpha (Uniprot: Q61727)

Reactivity Notes

Does not react with Human.

Localization

Cell Surface

Specificity

This monoclonal antibody recognizes Mouse Interleukin-10 receptor 1 (IL-10R1), a cell surface glycoprotein of approximately 110KDa. Its epitope is localized in the ligand-binding domain in the extracellular region of Mouse IL-10R1. This antibody blocks the binding of IL-10 to its receptor. IL-10R1 is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Binding of IL-10 to its receptor initiates JAK/STAT signaling that leads to B cell proliferation and inhibition of macrophage activation.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08221)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for IL-10R alpha Antibody (1B1.3a)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Neutralization Studies: Order Ab without Azide.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IL-10 R alpha

The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to tyrosine phosphorylation of JAK1 and Tyk2, but not JAK2 or JAK3. In addition to its role as an antagonist of IFN-gamma-mediated macrophage activation, IL-10Ralpha transmits differentiation as well as proliferative signals. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

Long Name

Interleukin 10 Receptor alpha

Alternate Names

CDw210a, IL-10Ra, IL10R alpha, IL10RA

Gene Symbol

IL10RA

UniProt

Q61727

Additional IL-10 R alpha Products

Product Documents for IL-10R alpha Antibody (1B1.3a)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IL-10R alpha Antibody (1B1.3a)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-10R alpha Antibody (1B1.3a)

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Protocols

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FAQs

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail