IL-10R alpha Antibody (1B1.3a) [Biotin]

Novus Biologicals | Catalog # NBP3-08221B

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Human (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Biotin

Antibody Source

Monoclonal Rat IgG1 kappa Clone # 1B1.3a
View all IL-10 R alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant extracellular region of Mouse IL-10R alpha (Uniprot: Q61727)

Reactivity Notes

Does not react with Human.

Localization

Cell Surface

Specificity

This monoclonal antibody recognizes Mouse Interleukin-10 receptor 1 (IL-10R1), a cell surface glycoprotein of approximately 110KDa. Its epitope is localized in the ligand-binding domain in the extracellular region of Mouse IL-10R1. This antibody blocks the binding of IL-10 to its receptor. IL-10R1 is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Binding of IL-10 to its receptor initiates JAK/STAT signaling that leads to B cell proliferation and inhibition of macrophage activation.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Applications for IL-10R alpha Antibody (1B1.3a) [Biotin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-10 R alpha

The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to tyrosine phosphorylation of JAK1 and Tyk2, but not JAK2 or JAK3. In addition to its role as an antagonist of IFN-gamma-mediated macrophage activation, IL-10Ralpha transmits differentiation as well as proliferative signals. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

Long Name

Interleukin 10 Receptor alpha

Alternate Names

CDw210a, IL-10Ra, IL10R alpha, IL10RA

Gene Symbol

IL10RA

Additional IL-10 R alpha Products

Product Documents for IL-10R alpha Antibody (1B1.3a) [Biotin]

Certificate of Analysis

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Product Specific Notices for IL-10R alpha Antibody (1B1.3a) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail