IL-17/IL-17A Antibody (4H1524)

Novus Biologicals | Catalog # NBP2-27338

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Block/Neutralize, Flow Cytometry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4H1524
View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 1-100 was used as immunogen

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for IL-17/IL-17A Antibody (4H1524)

Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338]

Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338]

Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using NBP2-27338 at 1 ug/ml.
Flow Cytometry: IL-17/IL-17A Antibody (4H1524) [NBP2-27338]

Flow Cytometry: IL-17/IL-17A Antibody (4H1524) [NBP2-27338]

Flow Cytometry: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using the PE conjugate of NBP2-25229. IL-17A positive cells in PMA/IM stimulated fresh PBMC: PBMC stimulated for 6h with PMA (25ng/ml) & IM (250ng/ml), in the presence of BFA. Cells were surface stained with CD4 APC, fixed & permeabilised for intracellular staining with PE conjugated
Block/Neutralize: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using Azide Free version of NBP2-27338. Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody

Applications for IL-17/IL-17A Antibody (4H1524)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Western Blot

1-3ug/ml~

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-17/IL-17A

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-Beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterzed and highly expressed by activated effector memory T cells.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for IL-17/IL-17A Antibody (4H1524)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IL-17/IL-17A Antibody (4H1524)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-17/IL-17A Antibody (4H1524)

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FAQs for IL-17/IL-17A Antibody (4H1524)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A:

    Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.

    A:

    Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A:

    Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.

    A:

    Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

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