IL-17/IL-17A Antibody (CL10252) - BSA Free

Novus Biologicals | Catalog # NBP3-07997

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10252

Format

BSA Free
View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q16552, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IL-17/IL-17A Antibody (CL10252) - BSA Free

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997]

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997]

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997] - Staining of human rectum shows moderate cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997]

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997]

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997]

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997]

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997] - Staining of human lymph node shows moderate cytoplasmic positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997]

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997]

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (CL10252) [NBP3-07997] - Staining of human urinary bladder shows strong cytoplasmic positivity in lymphoid cells.

Applications for IL-17/IL-17A Antibody (CL10252) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-17/IL-17A

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by TCRalpha/beta+ CD4 CD8 thymocytes, as well as by activated CD4+ and CD4+ CD45RO+ memory T cells (1). IL-17 stimulates the expression of TNFalpha, IL-1 beta, IL-6, and IL-8 (2). In addition, IL-17 is involved in the induction of inducible nitric oxide synthase and cyclooxygenase 2 in chondrocytes (3). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis (4).

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for IL-17/IL-17A Antibody (CL10252) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IL-17/IL-17A Antibody (CL10252) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-17/IL-17A Antibody (CL10252) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A:

    Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.

    A:

    Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A:

    Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.

    A:

    Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Showing  1 - 2 of 2 FAQs Showing All
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