IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.
Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-06804
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, cell supernatant, and other biological fluids (100 ul)
Sensitivity
46.88 pg/mL
Assay Range
78.13 - 5000 pg/mL
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Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Rat
Specificity
This kit recognizes Rat IL-1ra/IL-1F3/IL1RN in samples. No significant cross-reactivity or interference between Rat IL-1ra/IL-1F3/IL1RN and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) 4.51
Inter-Assay Precision (Precision between assays) 5.66
Recovery for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)
Recovery
86 - 105 %
Linearity
Scientific Data Images for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)
ELISA: Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric) [NBP3-06804] -
ELISA: Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric) [NBP3-06804] - Standard Curve ReferenceKit Contents for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: IL-1ra/IL-1F3
Long Name
Interleukin 1 Receptor Antagonist
Alternate Names
DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4
Gene Symbol
IL1RN
Additional IL-1ra/IL-1F3 Products
Product Documents for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
