IL-3 Antibody Pair [Biotin]

Novus Biologicals | Catalog # NBP3-57750

Novus Biologicals
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Key Product Details

Assay Type

Sandwich ELISA

Sample Type

Cell culture supernatants from cattle (Bos taurus). Additional biological sample types reported in the literature, including serum, plasma, peripheral blood mononuclear cell (PBMC) culture supernatants, bone marrow cell cultures, and tissue lysates. (100 ul)

Reactivity

Bovine
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Product Specifications

Description

Contains a capture antibody, detection antibody, and recombinant protein standard for assay development. These antibodies have been validated to function together in an ELISA format using the provided standard. Optimal buffers, antibody concentrations, incubation times, temperatures, and assay conditions have not been predetermined, allowing researchers to optimize assay sensitivity and dynamic range based on their specific sample type and experimental requirements. The quantities of components are not matched, and individual components may be purchased separately. This product is for research use only.

Sample Volume Required

100 ul

Conjugate

Biotin

Scientific Data Images for IL-3 Antibody Pair [Biotin]

IL-3 Antibody Pair [Biotin]

ELISA: IL-3 Antibody Pair [Biotin] [NBP3-57750] -

Representative standard curve for IL-3 Antibody Pair [Biotin] (NBP3-57750)

Kit Contents for IL-3 Antibody Pair [Biotin]

  • Capture Antibody (1 Set = <= 10 Plates; 2 Set = <= 20 Plates): (Catalog # <a href= "/products/il-3-antibody_nbp3-57651" >NBP3-57651</a>)
  • Detection Antibody (Biotinylated): (Catalog # <a href= "/products/il-3-antibody_nbp3-57682" >NBP3-57682</a>)
  • Standard: (Catalog # <a href= "/products/il-3-recombinant-protein_nbp3-57462" >NBP3-57462</a>)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-3

IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. Recombinant human IL-3 is a 17 kDa globular protein consisting of 133 amino acid residues.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Gene Symbol

IL3

Additional IL-3 Products

Product Documents for IL-3 Antibody Pair [Biotin]

Certificate of Analysis

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Product Specific Notices for IL-3 Antibody Pair [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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