Interleukin 36 alpha (IL-36 alpha), also known as IL-1F6 and FIL1 epsilon, is a member of the IL-1 family of proteins. Cells reported to express IL-36 alpha include monocytes, B cells, and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Recombinant IL-36 alpha has been shown to activate pathways involving NF-kappa B and MAPK in an IL-1 R6/IL-1 R rp2-dependent manner.
IL-36 alpha/IL-1F6 Antibody [FITC]
Novus Biologicals | Catalog # AF2297F
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA, Blockade of Receptor-ligand Interaction
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse IL‑36 alpha /IL‑1F6
Met1-His160
Accession # Q9JLA2
Met1-His160
Accession # Q9JLA2
Specificity
Detects mouse IL‑36 alpha /IL‑1F6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human (rh) IL-1F5, rhIL-1F6, and rhIL-1F9 is observed, and less than 2% cross-reactivity with recombinant mouse IL‑1F8, rhIL-1F7, rhIL-1 alpha, rhIL-1 beta, rhIL-1ra, and rhIL-18 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for IL-36 alpha/IL-1F6 Antibody [FITC]
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IL-36 alpha/IL-1F6
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Alternate Names
FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A
Gene Symbol
IL36A
Additional IL-36 alpha/IL-1F6 Products
Product Documents for IL-36 alpha/IL-1F6 Antibody [FITC]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-36 alpha/IL-1F6 Antibody [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
