Q: What is the Lyophilized buffer formulation (e.g. Lyophilized from PBS.)

A: This product is lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions

Recombinant Mouse IL-4 Protein

Novus Biologicals | Catalog # NBP2-35131

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Citations (5)

Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Accession Number

P07750

Applications

Bioactivity, SDS-PAGE

View all IL-4 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 121 amino acids corresponding to IL-4 Source: E. coli

Uniprot ID: P07750

Amino Acid Sequence: MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-4 as determined by LAL method.

Predicted Molecular Mass

13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-4 protein is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant prolifiration of Murine HT-2 cells is less then 2 ng/ml, corresponding to a Specific Activity of > 5 x 10^5 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-4 Protein

SDS-PAGE: Recombinant Mouse IL-4 Protein [NBP2-35131]

SDS-Page: Mouse IL-4 Protein [NBP2-35131]

Formulation, Preparation, and Storage

NBP2-35131

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-4

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. IL-4 enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The mouse IL-4 has a compact, globular fold, stabilised by 3 disulphide bonds and contains 121 amino acids residues which is a single non-glycosylated polypeptide. The human IL-4 shares about 40 % a.a. sequence identity with mouse/rat IL-4 and they are species-specific in their activities.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Gene Symbol

IL4

UniProt

P07750

Additional IL-4 Products

Product Documents for Recombinant Mouse IL-4 Protein

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Mouse IL-4 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Cytokines and Receptors Research

Dendritic Cell Development

gamma delta T Cells

Group 2 ILCs

Inflammatory Mediators

Microglia Activation Markers

Natural Killer T (NKT) Cells

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Citations for Recombinant Mouse IL-4 Protein

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FAQs for Recombinant Mouse IL-4 Protein

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Q: What is the Lyophilized buffer formulation (e.g. Lyophilized from PBS.)

A: This product is lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions