Recombinant Rat IL-4 Protein

Name: Recombinant Rat IL-4 Protein
Brand: Novus Biologicals
SKU: NBP2-35252
Price: 165 USD
Availability: InStock
Rating: 5 (1 reviews)

Novus Biologicals | Catalog # NBP2-35252

(1)

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Accession Number

P20096

Applications

Bioactivity, SDS-PAGE

View all IL-4 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 125 amino acids corresponding to IL-4 Source: E. coli

Uniprot ID: P20096

Amino Acid Sequence: HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-4 as determined by LAL method.

Predicted Molecular Mass

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL4 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat splenocytes is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBP2-35252 in the following applications:

In vitro bioactivity in cell culture (1 Review)

Scientific Data Images for Recombinant Rat IL-4 Protein

SDS-PAGE: Recombinant Rat IL-4 Protein [NBP2-35252]

SDS-Page: Rat IL-4 Protein [NBP2-35252]

Bioactivity: Recombinant Rat IL-4 Protein [NBP2-35252] - Gene expression of macrophages. Culturing with IL-4 increased Arg1 without affecting iNOS or IL-6. Rat macrophages were cultured with M-CSF. After maturation (7 days) in vitro, macrophages were cultured with with IL-4 + M-CSF or M-CSF alone. Changes in gene expression were assayed using RT-PCR using Image from verified customer review.

Formulation, Preparation, and Storage

NBP2-35252

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 5% trehalose.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-4

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. IL-4 can enhance both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The rat IL-4 shares about 40 % ~ 45 % a.a. sequence identity with human, mouse, rhesus macaque IL-4 and they are species-specific in their activities.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Gene Symbol

IL4

UniProt

P20096

Additional IL-4 Products

Product Documents for Recombinant Rat IL-4 Protein

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Rat IL-4 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Cytokines and Receptors Research

Dendritic Cell Development

gamma delta T Cells

Group 2 ILCs

Inflammatory Mediators

Microglia Activation Markers

Natural Killer T (NKT) Cells

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Customer Reviews for Recombinant Rat IL-4 Protein (1)

5 out of 5

1 Customer Rating

5 Stars

100%

4 Stars

3 Stars

2 Stars

1 Stars

Have you used Recombinant Rat IL-4 Protein?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Rat IL-4 Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 07/31/2018

Gene expression of macrophages. Culturing with IL-4 increased Arg1 without affecting iNOS or IL-6

Rat macrophages were cultured with M-CSF. After maturation (7 days) in vitro, macrophages were cultured with with IL-4 + M-CSF or M-CSF alone. Changes in gene expression were assayed using RT-PCR using commercial Taqman probes.