IMP2/IGF2BP2 Antibody (2O3W8)
Novus Biologicals | Catalog # NBP3-16585
Recombinant Monoclonal Antibody
![Western Blot: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]](https://resources.rndsystems.com/images/products/IMP2-IGF2BP2-Antibody-2O3W8-Western-Blot-NBP3-16585-img0002.jpg "Western Blot: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]")
Loading...
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2O3W8 expressed in HEK293
Loading...
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 500-595 of human IMP2/IGF2BP2 (NP_001007226.1). NFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPRDQTPDENEEVIVRIIGHFFASQTAQRKIREIVQQVKQQEQKYPQGVAS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IMP2/IGF2BP2 Antibody (2O3W8)
Western Blot: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]
Western Blot: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585] - Western blot analysis of extracts of various cell lines, using IGF2BP2/IMP2/IGF2BP2 Rabbit mAb (NBP3-16585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.![Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]](https://resources.rndsystems.com/images/products/IMP2-IGF2BP2-Antibody-2O3W8-Immunohistochemistry-Paraffin-NBP3-16585-img0001.jpg "Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]")
Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585]
Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585] - Immunohistochemistry of paraffin-embedded human colon using IGF2BP2/IMP2/IGF2BP2 Rabbit mAb (NBP3-16585) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585] -
Immunoprecipitation: IMP2/IGF2BP2 Antibody (2O3W8) [NBP3-16585] - Immunoprecipitation analysis of 300 ug extracts of HepG2 cells using 3 ug IMP2/IGF2BP2 antibody. Western blot was performed from the immunoprecipitate using IMP2/IGF2BP2 antibody at a dilution of 1:1000.Applications for IMP2/IGF2BP2 Antibody (2O3W8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IMP2
Long Name
Insulin-like Growth Factor 2 mRNA Binding Protein 2
Alternate Names
IF2B2, IGF2BP2, VICKZ2
Gene Symbol
IGF2BP2
Additional IMP2 Products
Product Documents for IMP2/IGF2BP2 Antibody (2O3W8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IMP2/IGF2BP2 Antibody (2O3W8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for IMP2/IGF2BP2 Antibody (2O3W8)
There are currently no reviews for this product. Be the first to review IMP2/IGF2BP2 Antibody (2O3W8) and earn rewards!
Have you used IMP2/IGF2BP2 Antibody (2O3W8)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...