IMPACT Antibody - BSA Free

Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221]

Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221]

Immunohistochemistry-Paraffin: IMPACT Antibody [NBP1-86221] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.

Western Blot: IMPACT Antibody - BSA Free [NBP1-86221]

Analysis in control (vector only transfected HEK293T lysate) and IMPACT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Western Blot: IMPACT Antibody - BSA Free [NBP1-86221] -

IMPACT overexpression confers GL261 glioma cells increased survival during tryptophan deprivation. a) Western blotting with indicated antibodies on GL261-wild-type and GL261-IMPACThigh lines cultured in standard media containing 50 μM tryptophan over a period of 8 days. Doubling time is shown as a mean +/- SD. b) Fluorescence microscopy images (20x objective) of GL261-wild-type and GL261-IMPACThigh lines stained with fluorescein diacetate and propidium iodide after five days of culture in media containing tryptophan concentrations ranging from 2.5 μM to 50 μM. c) Metabolic activity of GL261-wild-type and GL261-IMPACThigh lines determined using the MTT assay. Bar heights and whiskers represent a mean +/- SD of four replicates. Statistical significance was determined using Student’s t-test in Graphpad Prism v7.03. ***P < 0.001, **P < 0.01, ns P > 0.25 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30466445), licensed under a CC-BY license. Not internally tested by Novus Biologicals.