Influenza A H1N1/H3N2 M1 Antibody (GA2B) - (A/Puerto Rico/8/1934), (A/Bangkok/1/1979) - BSA Free

Novus Biologicals | Catalog # NB100-66552

Novus Biologicals

Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GA2B

Format

BSA Free
View all Influenza A H1N1/H3N2 M1 Primary Antibodies »

Product Specifications

Immunogen

Influenza A / Puerto Rico / 8 / 34 (H1N1) and A/Bangkok / 1 / 79 (H3N2) viruses

Specificity

Influenza A H1N1/H3N2 M1. (A/Puerto Rico/8/1934) (A/Bangkok/1/1979). Recognizes an epitope within the Influenza A matrix protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza A H1N1/H3N2 M1

Influenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions.

Additional Influenza A H1N1/H3N2 M1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Influenza A H1N1/H3N2 M1 Antibody (GA2B) - (A/Puerto Rico/8/1934), (A/Bangkok/1/1979) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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  • Q: I am looking for an antibody to be used against the influenza A M1 (matrix) protein of the PR8 strain, both in western blot and immunofluorescence microscopy. Your antibody NB100-66552 appears to be well suited for this. However, I want to use it for mutant proteins that differ in amino acids 97 and 100. Both these amino acids are located on the surface of the intact M1 protein. If the antibody binds near these amino acids, a difference in binding efficiency might occur that could influence my results. Therefore, I was wondering if you know to which part of the M1 protein this antibody binds.

    A:

    The lab has informed me that the epitope has not been mapped. I am very sorry that we do not have any helpful information! (We unfortunately do no epitope map our antibodies. All specific interactions and bindings are determined by experiment and will be listed in the Specificity section on our datasheet. If the specificity you are looking for is not listed, it has not yet been tested. If would like to try any of our antibodies in your experiment utilizing untested applications or species, you would qualify for our Innovators Reward Program.

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