Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89960

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Influenza A Virus H1N1

Applications

Western Blot, ELISA, Sandwich ELISA Detection

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 107 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Influenza A H1N1 Hemagglutinin (Accession#: ABD77675.1; Met 1-Gln 528).

Reactivity Notes

Influenza A H1N1

Specificity

No cross-reactivity in ELISA with: H1N1 (A/Brevig Mission/1/1918) HAH1N1 (A/Brisbane/02/2018) HAH1N1 (A/California/04/2009) HAH1N1 (A/California/07/2009) HAH1N1 (A/Guangdong-Maonan/SWL1536/2019) / (A/Hawaii/70/2019) HAH1N1 (A/Michigan/45/2015) HAH1N1 (A/New Caledonia/20/1999) HAH1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) HAH2N2 (A/Canada/720/2005) HAH3N2 (A/Brisbane/10/2007) HAH3N2 (A/Cambodia/e0826360/2020) HAH3N2 (A/Hong Kong/2671/2019) HAH3N2 (A/Hong Kong/45/2019) HAH3N2 (A/Hong Kong/4801/2014) HAH3N2 (A/Kansas/14/2017) HAH3N2 (A/Singapore/INFIMH-16-0019/2016) HAH3N2 (A/Switzerland/9715293/2013) HAH4N4 (A/mallard/Ohio/657/2002) HAH5N1 (A/Anhui/1/2005) HAH6N1 (A/northern shoveler/California/HKWF115/2007) HAH7N7 (A/chicken/Netherlands/1/03) HAH8N4 (A/pintail duck/Alberta/114/1979) HAH9N2 (A/Hong Kong/1073/99) HAH10N3 (A/duck/Hong Kong/786/1979) HAH11N2 (A/duck/Yangzhou/906/2002) HAH12N5 (A/green-winged teal/ALB/199/1991) HAH13N8 (A/black-headed gull/Netherlands/1/00) HAH15N8 (A/duck/AUS/341/1983) HAH16N3 (A/black-headed gull/Sweden/5/99) HAInfluenza B (B/Brisbane/60/2008) HAInfluenza B (B/Colorado/06/2017) HAInfluenza B (B/Florida/4/2006) HAInfluenza B (B/Florida/4/2006) HAInfluenza B (B/PHUKET/3073/2013) HAInfluenza B (B/Washington/02/2019) HA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free

Influenza A H1N1 Hemagglutinin Antibody (107) Western Blot: Influenza A H1N1 Hemagglutinin Antibody (107) [NBP2-89960]

Western Blot: Influenza A H1N1 Hemagglutinin Antibody (107) [NBP2-89960]

NBP2-89960 used at 1:1000 dilution. Lane A: H3N2 (A/Darwin/9/2021) HA Protein. Lane B: H3N2 (A/Darwin/9/2021) HA Protein. Lane C: H3N2 (A/Darwin/6/2021) HA Protein. Lane D: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein. Lane E: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.

Applications for Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Sandwich ELISA Detection

1:1000-1:10000

Western Blot

1:500-1:2000
Application Notes
This antibody can be used with the appropriate secondary reagents to detect Influenza A H1N1 Hemagglutinin - (A/Puerto Rico/8/1934) HA in Western Blot. Using a DAB detection system, the detection limit for Influenza A H1N1 Hemagglutinin - (A/Puerto Rico/8/1934) is approximately 5 ng/lane under non-reducing conditions and reducing conditions. The paired capture antibody is NBP2-89959.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Influenza A H1N1 Hemagglutinin Antibody (107) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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