Influenza A H1N1 PA Antibody (HL1388) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25529
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Virus
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1388 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-term region of Influenza A H1N1 PA (A/WSN/1933(H1N1)). The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Influenza A H1N1 PA Antibody (HL1388) - Azide and BSA Free
Western Blot: Influenza A H1N1 PA Antibody (HL1388) - Azide and BSA Free [NBP3-25529] -
Western Blot: Influenza A H1N1 PA Antibody (HL1388) - Azide and BSA Free [NBP3-25529] - Influenza A viral lysate and Influenza B viral lysate (0.5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody [HL1388] (NBP3-25529) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Influenza A H1N1 PA Antibody (HL1388) - Azide and BSA Free [NBP3-25529] -
Immunocytochemistry/Immunofluorescence: Influenza A H1N1 PA Antibody (HL1388) - Azide and BSA Free [NBP3-25529] - Influenza A virus PA protein antibody [HL1388] detects Influenza A virus PA protein protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Influenza A virus PA protein stained by Influenza A virus PA protein antibody [HL1388] (NBP3-25529) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: Influenza A H1N1 PA Antibody (HL1388) [NBP3-25529] -
Non-infected (-) and infected (+) H1299 whole cell extracts (1 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A H1N1 PA antibody [HL1388] (NBP3-25529) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Influenza A H1N1 PA Antibody (HL1388) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Influenza A H1N1 PA
Alternate Names
Influenza PA
Additional Influenza A H1N1 PA Products
Product Documents for Influenza A H1N1 PA Antibody (HL1388) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Influenza A H1N1 PA Antibody (HL1388) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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