Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Novus Biologicals | Catalog # NBP2-42874

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Porcine

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Influenza A H1N1 PA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus PA (A/WSN/1933(H1N1)). The exact sequence is proprietary.

Reactivity Notes

Influenza A Virus H1N1.

Specificity

Influenza A H1N1 PA (A/WSN/1933)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Western Blot: Influenza A H1N1 PA Antibody(A/WSN/1933) [NBP2-42874]

Western Blot: Influenza A H1N1 PA Antibody(A/WSN/1933) [NBP2-42874]

Western Blot: Influenza A H1N1 PA Antibody - (A/WSN/1933) [NBP2-42874] - Analysis of 60 ug whole cell lysate. A: DF-1(WSN infected cell lysates) B: DF-1 cell lysates diluted at 1:250.
Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Western Blot: Influenza A H1N1 PA Antibody - (A/WSN/1933) [NBP2-42874] -

Non-infected (-) and infected (+) H1299 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody (NBP2-42874) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 PA Antibody - (A/WSN/1933) [NBP2-42874] -

Influenza A virus PA protein antibody detects Influenza A virus PA protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus PA protein stained by Influenza A virus PA protein antibody (NBP2-42874) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Western Blot: Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free [NBP2-42874] -

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Western Blot: Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free [NBP2-42874] -

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Western Blot: Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free [NBP2-42874] -

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Influenza A H1N1 PA

Alternate Names

Influenza PA

Additional Influenza A H1N1 PA Products

Product Documents for Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

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Protocols

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FAQs

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