Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934)
Novus Biologicals | Catalog # NBP2-42875
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Key Product Details
Species Reactivity
Virus
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PA (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.
Specificity
Influenza A H1N1 PA (A/Puerto Rico/8/1934)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934)
Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934) [NBP2-42875]
Immunocytochemistry/Immunofluorescence: Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934) [NBP2-42875] - Analysis of A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: PA protein stained by Influenza A H1N1 PA antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37 degrees C 30 min.Western Blot: Influenza A H1N1 PA Antibody(A/Puerto Rico/8/1934) [NBP2-42875]
Western Blot: Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934) [NBP2-42875] - Analysis of 5 ug whole cell lysate. A: DF1 B: WSN virus infected DF1 10hr 7.5% SDS PAGE diluted at 1:5000.Western Blot: Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934) [NBP2-42875] -
Western Blot: Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934) [NBP2-42875] - Non-infected (-) and infected (+) H1299 whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A H1N1 PA antibody (NBP2-42875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Influenza A H1N1 PA
Alternate Names
Influenza PA
Additional Influenza A H1N1 PA Products
Product Documents for Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for Influenza A H1N1 PA Antibody - (A/Puerto Rico/8/1934)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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