Influenza A H1N1 PB2 Antibody - BSA Free

Western Blot: Influenza A H1N1 PB2 Antibody [NBP2-42879] -

Non-infected (-) and infected (+) H1299 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB2 protein antibody (NBP2-42879) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Influenza A H1N1 PB2 Antibody - BSA Free [NBP2-42879] -

Species-dependent support of ANP32A or ANP32B for Influenza A viral replication.Vectors carrying 20 ng of ANP32A or ANP32B proteins, or empty vectors, were co-transfected into DKO cells, together with a minigenome reporter, a Renilla expression control, and influenza virus polymerases from either avian influenza H7N9ZJ13 (A) or H9N2ZJ12 (B); human influenza WSN (C); swine influenza H1N1NC08 (D); canine influenza H3N2GD11 (E); or equine influenza H3N8XJ07 (F). Luciferase activity was measured 24 h later. (Data are Firefly activity normalized to Renilla, Statistical differences between cells are labeled according to a one-way ANOVA followed by a Dunnett’s test; NS = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The results represent at least three independent experiments.) ch, chicken; hu, human; sw, swine; eq, equine; ca, canine. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Influenza A H1N1 PB2 Antibody - BSA Free [NBP2-42879] -

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Influenza A H1N1 PB2 Antibody - BSA Free [NBP2-42879] -

Species-dependent support of ANP32A or ANP32B for Influenza A viral replication.Vectors carrying 20 ng of ANP32A or ANP32B proteins, or empty vectors, were co-transfected into DKO cells, together with a minigenome reporter, a Renilla expression control, and influenza virus polymerases from either avian influenza H7N9ZJ13 (A) or H9N2ZJ12 (B); human influenza WSN (C); swine influenza H1N1NC08 (D); canine influenza H3N2GD11 (E); or equine influenza H3N8XJ07 (F). Luciferase activity was measured 24 h later. (Data are Firefly activity normalized to Renilla, Statistical differences between cells are labeled according to a one-way ANOVA followed by a Dunnett’s test; NS = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The results represent at least three independent experiments.) ch, chicken; hu, human; sw, swine; eq, equine; ca, canine. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Influenza A H1N1 PB2 Antibody - BSA Free [NBP2-42879] -

Species-dependent support of ANP32A or ANP32B for Influenza A viral replication.Vectors carrying 20 ng of ANP32A or ANP32B proteins, or empty vectors, were co-transfected into DKO cells, together with a minigenome reporter, a Renilla expression control, and influenza virus polymerases from either avian influenza H7N9ZJ13 (A) or H9N2ZJ12 (B); human influenza WSN (C); swine influenza H1N1NC08 (D); canine influenza H3N2GD11 (E); or equine influenza H3N8XJ07 (F). Luciferase activity was measured 24 h later. (Data are Firefly activity normalized to Renilla, Statistical differences between cells are labeled according to a one-way ANOVA followed by a Dunnett’s test; NS = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The results represent at least three independent experiments.) ch, chicken; hu, human; sw, swine; eq, equine; ca, canine. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Influenza A H1N1 PB2 Antibody - BSA Free [NBP2-42879] -

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Influenza A H1N1 PB2 Antibody - BSA Free [NBP2-42879] -

Species-dependent support of ANP32A or ANP32B for Influenza A viral replication.Vectors carrying 20 ng of ANP32A or ANP32B proteins, or empty vectors, were co-transfected into DKO cells, together with a minigenome reporter, a Renilla expression control, and influenza virus polymerases from either avian influenza H7N9ZJ13 (A) or H9N2ZJ12 (B); human influenza WSN (C); swine influenza H1N1NC08 (D); canine influenza H3N2GD11 (E); or equine influenza H3N8XJ07 (F). Luciferase activity was measured 24 h later. (Data are Firefly activity normalized to Renilla, Statistical differences between cells are labeled according to a one-way ANOVA followed by a Dunnett’s test; NS = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The results represent at least three independent experiments.) ch, chicken; hu, human; sw, swine; eq, equine; ca, canine. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Influenza A H1N1 PB2 Antibody - BSA Free [NBP2-42879] -

Species-dependent support of ANP32A or ANP32B for Influenza A viral replication.Vectors carrying 20 ng of ANP32A or ANP32B proteins, or empty vectors, were co-transfected into DKO cells, together with a minigenome reporter, a Renilla expression control, and influenza virus polymerases from either avian influenza H7N9ZJ13 (A) or H9N2ZJ12 (B); human influenza WSN (C); swine influenza H1N1NC08 (D); canine influenza H3N2GD11 (E); or equine influenza H3N8XJ07 (F). Luciferase activity was measured 24 h later. (Data are Firefly activity normalized to Renilla, Statistical differences between cells are labeled according to a one-way ANOVA followed by a Dunnett’s test; NS = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The results represent at least three independent experiments.) ch, chicken; hu, human; sw, swine; eq, equine; ca, canine. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Influenza A H1N1 PB2 Antibody - BSA Free [NBP2-42879] -

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Influenza A H1N1 PB2 Antibody - BSA Free [NBP2-42879] -

Species-dependent support of ANP32A or ANP32B for Influenza A viral replication.Vectors carrying 20 ng of ANP32A or ANP32B proteins, or empty vectors, were co-transfected into DKO cells, together with a minigenome reporter, a Renilla expression control, and influenza virus polymerases from either avian influenza H7N9ZJ13 (A) or H9N2ZJ12 (B); human influenza WSN (C); swine influenza H1N1NC08 (D); canine influenza H3N2GD11 (E); or equine influenza H3N8XJ07 (F). Luciferase activity was measured 24 h later. (Data are Firefly activity normalized to Renilla, Statistical differences between cells are labeled according to a one-way ANOVA followed by a Dunnett’s test; NS = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The results represent at least three independent experiments.) ch, chicken; hu, human; sw, swine; eq, equine; ca, canine. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.