Influenza B Hemagglutinin Antibody (10H6C8G4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-06569

Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10H6C8G4

Format

Azide and BSA Free
View all Influenza B Hemagglutinin Primary Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza B Hemagglutinin (Accession#: ACA33493.1; Met 1-Ala 555).

Reactivity Notes

No cross-reactivity in ELISA with:

H1N1 (A/Brisbane/59/2007) HA
H2N2 (A/Canada/720/2005) HA
H3N2 (A/Brisbane/10/2007) HA
H4N4 (A/mallard/Ohio/657/2002) HA
H5N1 (A/Anhui/1/2005) HA
H5N8 (A/northern shoveler/California/HKWF115/2007) HA
H7N7 (A/Netherlands/219/03) HA
H8N4 (A/pintail duck/Alberta/114/1979) HA
H9N2 (A/Hong Kong/1073/99) HA
H10N3 (A/duck/Hong Kong/786/1979) HA
H11N2 (A/duck/Yangzhou/906/2002) HA
H12N5 (A/green-winged teal/ALB/199/1991) HA
H13N8 (A/black-headed gull/Netherlands/1/00) HA
H15N8 (A/duck/AUS/341/1983) HA
H16N3 (A/black-headed gull/Sweden/5/99) HA

Specificity

Has cross-reactivity in ELISA with: Influenza B (B/Brisbane/60/2008) HAInfluenza B (B/Colorado/06/2017) HAInfluenza B (B/PHUKET/3073/2013) HAInfluenza B (B/Washington/02/2019) HANo cross-reactivity in ELISA with: H1N1 (A/Brisbane/02/2018) HAH1N1 (A/Brisbane/59/2007) HAH1N1 (A/California/04/2009) HAH1N1 (A/California/07/2009) HAH1N1 (A/Guangdong-Maonan/SWL1536/2019) / (A/Hawaii/70/2019) HAH1N1 (A/Michigan/45/2015) HAH1N1 (A/Puerto Rico/8/1934) HAH1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) HAH2N2 (A/Canada/720/2005) HAH3N2 (A/Brisbane/10/2007) HAH3N2 (A/Cambodia/e0826360/2020) HAH3N2 (A/Hong Kong/2671/2019) HAH3N2 (A/Hong Kong/45/2019) HAH3N2 (A/Hong Kong/4801/2014) HAH3N2 (A/Kansas/14/2017) HAH3N2 (A/Singapore/INFIMH-16-0019/2016) HAH3N2 (A/Switzerland/9715293/2013) HAH4N4 (A/mallard/Ohio/657/2002) HAH5N1 (A/Anhui/1/2005) HAH5N8 (A/northern shoveler/California/HKWF115/2007) HAH7N7 (A/Netherlands/219/03) HAH8N4 (A/pintail duck/Alberta/114/1979) HAH9N2 (A/Hong Kong/1073/99) HAH10N3 (A/duck/Hong Kong/786/1979) HAH11N2 (A/duck/Yangzhou/906/2002) HAH12N5 (A/green-winged teal/ALB/199/1991) HAH13N8 (A/black-headed gull/Netherlands/1/00) HAH15N8 (A/duck/AUS/341/1983) HAH16N3 (A/black-headed gull/Sweden/5/99) HA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Influenza B Hemagglutinin Antibody (10H6C8G4) - Azide and BSA Free

Western Blot: Influenza B Hemagglutinin Antibody (10H6C8G4) [NBP3-06569]

Western Blot: Influenza B Hemagglutinin Antibody (10H6C8G4) [NBP3-06569]

Western Blot: Influenza B Hemagglutinin Antibody (10H6C8G4) [NBP3-06569] - Anti-HA mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane A: H3N2 (A/Darwin/9/2021) HA Protein Lane B: H3N2 (A/Darwin/9/2021) HA ProteinLane C: H3N2 (A/Darwin/6/2021) HA ProteinLane D: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein Lane E: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA ProteinSecondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.
Influenza B Hemagglutinin Antibody (10H6C8G4)

ELISA: Mouse Monoclonal Influenza B Hemagglutinin Antibody (10H6C8G4) [NBP3-06569] -

ELISA: Mouse Monoclonal Influenza B Hemagglutinin Antibody (10H6C8G4) [NBP3-06569] - Analysis using HRP conjugated version of Mouse Monoclonal Influenza B Hemagglutinin Antibody (NBP3-06569H). The absorbance signal of antibody increases together with the weight of Influenza B (B/PHUKET/3073/2013) HA strain between 0.375 - 0.01 µg of HA range. Image from verified customer review.

Applications for Influenza B Hemagglutinin Antibody (10H6C8G4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Western Blot

1:500-1:2000
Application Notes
Western Blot: Using a DAB detection system, the detection limit for Influenza B Hemagglutinin is approximately 12.5 ng/lane under non-reducing conditions and reducing conditions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza B Hemagglutinin

Alternate Names

B1AG46

Additional Influenza B Hemagglutinin Products

Product Documents for Influenza B Hemagglutinin Antibody (10H6C8G4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Influenza B Hemagglutinin Antibody (10H6C8G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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