INO80 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-78759

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all INO80 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1506 and 1556 of human INO80 homolog using the numbering given in entry NP_060023.1 (GeneID 54617).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for INO80 Antibody - BSA Free

Western Blot: INO80 Antibody [NBP1-78759]

Western Blot: INO80 Antibody [NBP1-78759]

Western Blot: INO80 Antibody [NBP1-78759] - Detection of human INO80 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, A-549, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INO80 antibody NBP1-78759 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Western Blot: INO80 Antibody [NBP1-78759]

Western Blot: INO80 Antibody [NBP1-78759]

Western Blot: INO80 Antibody [NBP1-78759] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-INO80 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. INO80 was also immunoprecipitated by rabbit anti-INO80 antibody NBP1-78758 which recognizes an upstream epitope.
Immunoprecipitation: INO80 Antibody [NBP1-78759]

Immunoprecipitation: INO80 Antibody [NBP1-78759]

Immunoprecipitation: INO80 Antibody [NBP1-78759] - Detection of human INO80 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INO80 antibody lot NBP1-78759-2 used for IP at 6 ug per reaction. INO80 was also immunoprecipitated by a previous lot of this antibody NBP1-78759-1 and rabbit anti-INO80 antibody NBP1-78758-2. For blotting immunoprecipitated INO80, A303-371A was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for INO80 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: INO80

INOC1 is the human homolog of S. cerevisiae Ino80, the catalytic ATPase subunit of the INO80 chromatin remodeling complex (Shen et al., 2000 (PubMed 10952318); Bakshi et al., 2004 (PubMed 15207721)). INOC1 defines a subfamily of SWI2/SNF2 chromatin remodeling proteins; see SMARCA (MIM 600014) for background information.(supplied by OMIM)

Alternate Names

EC 3.6.1, EC 3.6.1.23, EC 3.6.4.12, hINO80INO80 complex subunit A, homolog of yeast INO80, Ino80, INO80 complex homolog 1 (S. cerevisiae), INO80 homolog (S. cerevisiae), INO80A, INOC1putative DNA helicase INO80 complex homolog 1, KIAA1259

Gene Symbol

INO80

UniProt

NP_060023.1 (Human)

Additional INO80 Products

Product Documents for INO80 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for INO80 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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