Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Label

CoraFluor 1

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 11
Phe19-Pro1141
Accession # P61622

Specificity

Detects mouse Integrin alpha 11 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) Integrin alpha 11 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Integrin  alpha 2 and rmIntegrin alpha 10 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for Integrin alpha 11 Antibody [CoraFluor™ 1]

Integrin alpha 11 Antibody [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Integrin alpha 11 Antibody [CoraFluor™ 1]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Integrin alpha 11

Integrin alpha-11 is a protein that functions as a heterodimer of an alpha and beta subunit, may be involved in attaching muscle tissue to the extracellular matrix, is most highly expressed in the uterus and heart, and is encoded by a gene comprised of 1188 amino acids with a weight of approximately 133 kDa. Studies are being conducted on several diseases and disorders related to this protein including ADHD, lung cancer, adenocarcinoma, thyroiditis, and prostatitis. Integrin alpha-11 has also been shown to have interactions with ITGB1, COL1A1, COL1A2, ACTB, and ACTG1 in pathways such as the focal adhesion, CDK5, NR-kappaB, integrin-mediated cell adhesion, signal transduction, and ECM-receptor interaction pathways.

Alternate Names

ITGA11

Gene Symbol

ITGA11

Additional Integrin alpha 11 Products

Product Documents for Integrin alpha 11 Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Integrin alpha 11 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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