Mouse Integrin  alpha 11 Antibody

Catalog # Availability Size / Price Qty
AF6498
AF6498-SP
Mouse Integrin  alpha 11 Antibody in Western Blot
2 Images
Product Details
Citations (1)
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Mouse Integrin  alpha 11 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 11 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) Integrin alpha 11 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Integrin  alpha 2 and rmIntegrin alpha 10 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 11
Phe19-Pro1141
Accession # P61622
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse Integrin  alpha 11 by Western Blot. View Larger

Detection of Mouse Integrin  alpha 11 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Integrin  alpha 11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin  alpha 11 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Integrin alpha 11 in Mouse Embryonic Fibroblasts (iMEF). View Larger

Integrin alpha 11 in Mouse Embryonic Fibroblasts (iMEF). Integrin alpha 11 was detected in immersion fixed irradiated mouse embryonic fibroblasts (iMEF) using Mouse Integrin alpha 11 Affinity-purified Polyclonal Antibody (Catalog # AF6498) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 11

Integrin alpha 11 ( ITGA11) is a 155-160 kDa member of the integrin alpha chain family of molecules. It preferentially forms a cell surface heterodimer with beta 1 Integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II, plus group A Streptococcal Scl1 protein. ITGA11 is expressed by embryonic mesenchymal cells in areas that incorporate collagen during development. In adult tissue, fibroblasts express ITGA11, and a collagen:ITGA11 interaction likely contributes to the formation of myofibroblasts. Mature mouse ITGA11 is an 1166 amino acid (aa) type I transmembrane glycoprotein. It contains a large 1119 aa extracellular domain (ECD) (aa 23-1141) plus a short 24 aa cytoplasmic region. The ECD shows 7 x 60 aa FG-Gap repeats that generate beta -propellers (aa 24-650) with an intervening I domain that binds collagen (aa 164-345). There is one potential splice variant that shows an eight aa substitution for aa 89-119 coupled to a seven aa insertion after Gly690. Over aa 23-1141, mouse ITGA11 shares 95% and 90% aa identity with rat and human ITGA11, respectively.

Entrez Gene IDs
22801 (Human); 319480 (Mouse); 315744 (Rat)
Alternate Names
HsT18964; Integrin alpha 11; integrin alpha-11; integrin, alpha 11; ITGA11

Product Datasheets

Citation for Mouse Integrin  alpha 11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Highlights of glycosylation and adhesion related genes involved in myogenesis.
    Authors: Grassot V, Da Silva A, Saliba J, Maftah A, Dupuy F, Petit J
    BMC Genomics, 2014;15(0):621.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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