|Detection of Mouse Integrin alpha 11 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Integrin alpha 11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin alpha 11 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
|Integrin alpha 11 in Mouse Embryonic Fibroblasts (iMEF). Integrin alpha 11 was detected in immersion fixed irradiated mouse embryonic fibroblasts (iMEF) using Mouse Integrin alpha 11 Affinity-purified Polyclonal Antibody (Catalog # AF6498) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
Integrin alpha 11 ( ITGA11) is a 155-160 kDa member of the integrin alpha chain family of molecules. It preferentially forms a cell surface heterodimer with beta 1 Integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II, plus group A Streptococcal Scl1 protein. ITGA11 is expressed by embryonic mesenchymal cells in areas that incorporate collagen during development. In adult tissue, fibroblasts express ITGA11, and a collagen:ITGA11 interaction likely contributes to the formation of myofibroblasts. Mature mouse ITGA11 is an 1166 amino acid (aa) type I transmembrane glycoprotein. It contains a large 1119 aa extracellular domain (ECD) (aa 23-1141) plus a short 24 aa cytoplasmic region. The ECD shows 7 x 60 aa FG-Gap repeats that generate beta -propellers (aa 24-650) with an intervening I domain that binds collagen (aa 164-345). There is one potential splice variant that shows an eight aa substitution for aa 89-119 coupled to a seven aa insertion after Gly690. Over aa 23-1141, mouse ITGA11 shares 95% and 90% aa identity with rat and human ITGA11, respectively.
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