Integrin alpha 4/CD49d Antibody (9F10) - BSA Free
Novus Biologicals | Catalog # NBP1-43398
Clone 9F10 was used by HLDA to establish CD designation.
![Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]](https://resources.rndsystems.com/images/products/Integrin-alpha-4-CD49d-Antibody-9F10-Flow-Cytometry-NBP1-43398-img0001.jpg "Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 9F10
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this antibody was CD49d.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse Integrin alpha 4/CD49d Antibody (9F10) - BSA Free (NBP1-43398) is a monoclonal antibody validated for use in IHC, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Integrin alpha 4/CD49d Antibody (9F10) - BSA Free
Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]
Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.125 ug of Anti-Human CD49d Purified (filled histogram) followed by Anti-Mouse IgG FITC. Cells in the lymphocyte gate were used for analysis.![Immunofluorescence: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]](https://resources.rndsystems.com/images/products/Integrin-alpha-4-CD49d-Antibody-9F10-Immunofluorescence-NBP1-43398-img0002.jpg "Immunofluorescence: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]")
Immunofluorescence: Integrin alpha 4/CD49d Antibody (9F10) - BSA Free [NBP1-43398]
Immunofluorescence: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Analysis was performed using 70% confluent log phase MOLT-4 cells.Applications for Integrin alpha 4/CD49d Antibody (9F10) - BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The 9F10 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 ug per test.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Integrin alpha 4/CD49d
Alternate Names
CD49d, ITGA4, VLA-4
Gene Symbol
ITGA4
Additional Integrin alpha 4/CD49d Products
Product Documents for Integrin alpha 4/CD49d Antibody (9F10) - BSA Free
Product Specific Notices for Integrin alpha 4/CD49d Antibody (9F10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Blood-Brain Barrier and Immune Cell Transmigration: Pathways Overview
Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways
Blood-Brain Barrier Pathway: Anatomy
