Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-26661

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry

Cited:

Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # PS/2

Format

Azide and BSA Free
View all Integrin alpha 4/CD49d Primary Antibodies »

Product Specifications

Immunogen

P815 cells

Specificity

CD49d, the alpha 4 chain (100 kDa) of the VLA-4 integrin heterodimer (Mr 150-kDa)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free

Immunohistochemistry-Paraffin: Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free [NBP1-26661]

Immunohistochemistry-Paraffin: Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free [NBP1-26661]

Immunohistochemistry-Paraffin: Integrin alpha 4/CD49d Antibody (PS/2) [NBP1-26661] - FFPE human ovarian carcinoma. Antibody at 1:10. HIER antigen retrieval at pH 6. HRP/DAB detetion. IHC-P image submitted by a verified customer review.
Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free [NBP1-26661]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free [NBP1-26661]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) [NBP1-26661] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD49d-UNLB followed by Mouse Anti-Rat IgG2b-AF647.

Applications for Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry-Paraffin

Validated from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP1-26661 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha 4/CD49d

VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors. (1-3) As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. (1, 2) In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. (2, 3) CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. (1, 2) Antibody PS/2 also reacts with human VLA-4. (1)

Alternate Names

CD49d, ITGA4, VLA-4

Entrez Gene IDs

3676 (Human)

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free

Customer Reviews for Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free (1)

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  • Integrin alpha 4/CD49d Antibody (PS/2)
    Name: Haopei Zhang
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human ovarian carcinoma
    Species: Human
    Verified Customer | Posted 01/04/2019
    NBP1-26661 staining integrin alpha 4 in human ovarian cancer tissue sample by Immunohistochemistry (Formalin-fixed paraffin-embedded sections)
    Dilution: 1:10 Antigen Retrieval: HIER in pressure cooker for 3 minutes with sodium citrate buffer (pH=6) Incubated at 4℃ overnight (16 hours) HRP/DAB detection system
    Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free NBP1-26661

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