Integrin alpha 4/CD49d Antibody (PS/2) [FITC]

Novus Biologicals | Catalog # NBP1-26662

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # PS/2
View all Integrin alpha 4/CD49d Primary Antibodies »

Product Specifications

Immunogen

P815 cells

Specificity

CD49d, the alpha 4 chain (100 kDa) of the VLA-4 integrin heterodimer (Mr 150-kDa)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for Integrin alpha 4/CD49d Antibody (PS/2) [FITC]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) [FITC] [NBP1-26662] - Lymph node cells from EAE mice stained for viable cells, VLA4 and CD4. CD4+, VLA4 non-stained cells (red) and CD4+, VLA-stained cells (blue). Image from verified customer review.
Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) [FITC] [NBP1-26662] - Less or equal to 2 ug/10^6 cellsCells from BALB/c bone marrow were stained with rat anti-mouse CD49d-FITC, following which lymphocytes were gated and analyzed on a flow cytometer.

Applications for Integrin alpha 4/CD49d Antibody (PS/2) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-26662 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha 4/CD49d

VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors. (1-3) As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. (1, 2) In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. (2, 3) CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. (1, 2) Antibody PS/2 also reacts with human VLA-4. (1)

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (PS/2) [FITC]

Certificate of Analysis

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Product Specific Notices for Integrin alpha 4/CD49d Antibody (PS/2) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Integrin alpha 4/CD49d Antibody (PS/2) [FITC] (1)

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Customer Images

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  • Integrin alpha 4/CD49d Antibody (PS/2) [FITC]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Lymph node cells
    Species: Mouse
    Verified Customer | Posted 04/04/2019
    Lymph node cells from EAE mice stained for viable cells, VLA4 and CD4. CD4+, VLA4 non-stained cells (red) and CD4+, VLA-stained cells (blue).
    Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and periaortic and inguinal lymph node cells were isolated when the animals were at the onset of symptoms. Cells were stained for VLA4, CD4, CD45, LFA-1, TCRb, CD28, CTLA-4, CD40L, OX40, PD1 and CD11b, plus for viability to exclude dead cells. VLA4 staining is showed in CD4+ cells from a control sample not stained for VLA4 (red) and an experimental sample stained for VLA.
    Integrin alpha 4/CD49d Antibody (PS/2) [FITC] NBP1-26662

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