Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # AP-MAB0828

Format

Azide and BSA Free
View all Integrin alpha E/CD103 Primary Antibodies »

Product Specifications

Immunogen

Mouse mammary tumor cells

Specificity

Integrin alpha IEL chain

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Applications for Integrin alpha E/CD103 Antibody (AP-MAB0828) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
Flow Cytometry, Immunoprecipitation, Immunohistochemistry-Frozen and Inhibition of CD103 mediated adhesion. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Integrin alpha E/CD103

CD103 has a unique and fairly restricted tissue distribution. It is expressed on almost all intestinal intraepithelial lymphocytes (IEL), on dendritic epidermal T cells (DEC), on subpopulations of peripheral T cells, and on distinct subsets of fetal, neonatal, and adult thymocytes. E-cadherin is the epithelial-cell ligand for CD103. The ordered expression of alpha IEL during thymocyte development (which occurs under the influence of the thymic epithelium), the high level of expression of alphaIEL on those peripheral T cells found in epithelial tissues (IEL and DEC), and the expression of CD103 on a subset of CD8+ lymphocytes responding to allogeneic epithelial cells suggest that CD103 may have a common role in the interactions of T lymphocytes with epithelia during T-cell maturation and effector functions. This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse mammary tumor cells.

Alternate Names

CD103, HUMINAE, ITGAE

Entrez Gene IDs

16407 (Mouse)

Gene Symbol

ITGAE

Additional Integrin alpha E/CD103 Products

Product Documents for Integrin alpha E/CD103 Antibody (AP-MAB0828) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Integrin alpha E/CD103 Antibody (AP-MAB0828) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Integrin alpha E/CD103 Antibody (AP-MAB0828) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Integrin alpha E/CD103 Antibody (AP-MAB0828) - Azide and BSA Free

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  • Q: We would be interested in the following antibody from your company: NBP1-06674 However, it is not exactly clear to us, what your company means when it says mouse mammary tumor cells were used as immunogen. Does this mean that tumor cells overexpressing CD103 were used? Since mouse mammary tumor cells per se are not really an appropriate immunogen.

    A: Our Integrin alpha E antibody NBP1-06674 was created using mouse mammary tumor cells known to overexpress the ITGAE protein. These were selected and cultured to produce this monoclonal. We fully guarantee this antibody to detect mouse ITGAE protein in FLOW, IHC-frozen tissues, and immunoprecipitation.

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