Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-08855
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free [NBP3-08855]](https://resources.rndsystems.com/images/products/Integrin-alpha-E-CD103-Antibody-ITGAE-3904R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08855-img0001.jpg "Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free [NBP3-08855]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # ITGAE/3904R
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human Integrin alpha E/CD103 protein fragment (aa 775-855) (exact sequence is proprietary) (Uniprot: P38570 )
Localization
Cell surface and cytoplasm
Marker
T-Cell Lymphoma & Hairy Cell Leukemia Marker
Specificity
This monoclonal antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07530).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free [NBP3-08855]
Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free [NBP3-08855] - Formalin-fixed, paraffin-embedded human Tonsil stained with Integrin alpha E/CD103 Recombinant Rabbit Monoclonal Antibody (ITGAE/3904R).Applications for Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Integrin alpha E/CD103
Alternate Names
CD103, HUMINAE, ITGAE
Gene Symbol
ITGAE
Additional Integrin alpha E/CD103 Products
Product Documents for Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Innate Lymphoid Cell Differentiation Pathways
