Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free
Novus Biologicals | Catalog # NBP1-41272
![Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]](https://resources.rndsystems.com/images/products/Integrin-beta-2-CD18-Antibody-M18-2-Low-Endotoxin-Flow-Cell-Surface-NBP1-41272-img0002.jpg "Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]")
Key Product Details
Species Reactivity
Mouse, Canine (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation, Functional, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # M18/2
Format
Azide and BSA Free
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Product Specifications
Immunogen
Murine cytotoxic T cell glycoproteins
Specificity
The rat monoclonal antibody M18/2 recognizes CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
Endotoxin level is less than 0.01 EU/ug of the protein
Scientific Data Images
Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]
Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin [NBP1-41272] - Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2) FITC.![Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]](https://resources.rndsystems.com/images/products/Integrin-beta-2-CD18-Antibody-M18-2-Low-Endotoxin-Flow-Cell-Surface-NBP1-41272-img0001.jpg "Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]")
Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]
Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin [NBP1-41272] - Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2).![Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free Flow Cytometry: Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]](https://resources.rndsystems.com/images/products/nbp1-41272_rat-monoclonal-integrin-beta-2-cd18-antibody-m18-2-low-endotoxin-13120241659351.jpg "Flow Cytometry: Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]")
Flow Cytometry: Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]
Separation of murine myeloid cells stained using anti-mouse CD18 (M18/2) purified antibody (low endotoxin, concentration in sample 16 ug/ml, DAR APC, red-filled) from murine myeloid cells unstained by primary antibody (DAR APC, black-dashed) in Analysis (surface staining) of murine splenocyte suspension.Applications
Application
Recommended Usage
Flow Cytometry
1 ug/ml
Immunohistochemistry-Frozen
1:200
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
1:50
Western Blot
1:1000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Integrin beta 2/CD18
Alternate Names
CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta
Gene Symbol
ITGB2
Additional Integrin beta 2/CD18 Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Blood-Brain Barrier and Immune Cell Transmigration: Pathways Overview
Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways
Blood-Brain Barrier Pathway: Anatomy
