integrin beta 4 binding protein Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03384

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all integrin beta 4 binding protein Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human integrin beta 4 binding protein (NP_852133.1). MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYSVFEGELSDTIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDTVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for integrin beta 4 binding protein Antibody - BSA Free

integrin beta 4 binding protein Antibody - BSA Free

Western Blot: integrin beta 4 binding protein Antibody - BSA Free [NBP3-03384] -

Western Blot: integrin beta 4 binding protein Antibody - BSA Free [NBP3-03384] - Western blot analysis of various lysates, using integrin beta 4 binding protein Rabbit pAb (A1818) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
integrin beta 4 binding protein Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: integrin beta 4 binding protein Antibody - BSA Free [NBP3-03384] -

Immunocytochemistry/ Immunofluorescence: integrin beta 4 binding protein Antibody - BSA Free [NBP3-03384] - Confocal immunofluorescence analysis of U-2OS cells using integrin beta 4 binding protein antibody (A1818) at dilution of 1:50. Blue: DAPI for nuclear staining.
integrin beta 4 binding protein Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: integrin beta 4 binding protein Antibody - BSA Free [NBP3-03384] -

Immunocytochemistry/ Immunofluorescence: integrin beta 4 binding protein Antibody - BSA Free [NBP3-03384] - Confocal immunofluorescence analysis of Hela cells using integrin beta 4 binding protein antibody (A1818) at dilution of 1:50. Blue: DAPI for nuclear staining.
integrin beta 4 binding protein Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: integrin beta 4 binding protein Antibody - BSA Free [NBP3-03384] -

Immunocytochemistry/ Immunofluorescence: integrin beta 4 binding protein Antibody - BSA Free [NBP3-03384] - Immunofluorescence analysis of MCF-7 cells using integrin beta 4 binding protein antibody (A1818). Blue: DAPI for nuclear staining.

Applications for integrin beta 4 binding protein Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: integrin beta 4 binding protein

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

b(2)gcn, B(2)GCN homolog, B4 integrin interactor, CAB, EIF3Ap27(BBP), eIF-6, eukaryotic translation initiation factor 3A, eukaryotic translation initiation factor 6, ITGB4BPintegrin beta 4 binding protein, p27 beta-4 integrin-binding protein, p27BBP

Gene Symbol

EIF6

Additional integrin beta 4 binding protein Products

Product Documents for integrin beta 4 binding protein Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for integrin beta 4 binding protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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