Ionomycin calcium salt

Tocris Bioscience | Catalog # 1704

Calcium ionophore
Tocris Bioscience

Key Product Details

Description

Calcium ionophore
View all Calcium Signaling Agents »

Product Description

Ionomycin calcium salt is a calcium ionophore; more specific than A23187.

Free Acid also available.

Product Specifications for Ionomycin calcium salt

Molecular Weight

747.08

Formula

C41H70O9.Ca

Storage

Store at -20°C

Purity

≥97% (HPLC)

Chemical Name

(4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Trihydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid calcium salt

CAS Number

56092-82-1

PubChem ID

6446270

InChI Key

WKRWUYKLUMMAKG-WYGBAUISSA-L

SMILES

O=C([O-])CC[C@@H](C)C[C@H](C)C[C@H](C)C(/C=C([O-])/[C@H](C)C[C@@H](C/C=C/[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]1CC[C@]([C@]2([H])O[C@@]([C@H](O)C)(C)CC2)(C)O1)C)=O.[Ca+2]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 18.68 25 with gentle warming
Ethanol 74.71 100

Preparing Stock Solutions for Ionomycin calcium salt

The following data is based on the product molecular weight 747.08.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.34 mL 6.69 mL 13.39 mL
5 mM 0.27 mL 1.34 mL 2.68 mL
10 mM 0.13 mL 0.67 mL 1.34 mL
50 mM 0.03 mL 0.13 mL 0.27 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 34 publications citing the usage of this product.

Product Documents for Ionomycin calcium salt

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ionomycin calcium salt

For research use only

Citations for Ionomycin calcium salt

Customer Reviews for Ionomycin calcium salt (3)

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Customer Images

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  • Good product for calcium imaging
    Name: Yuyang Sun
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HSG
    Verified Customer | Posted 05/26/2022
    Used it for calcium image as positive control. Get significant trace increase after application.
    Ionomycin calcium salt 1704
  • Ionomycin Ca depletes intracellular Ca stores.
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 01/11/2020
    HEK293 cells were loaded with intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 40 min in dark at room temperature, washed and kept in normal tyrode for 20 min. Fura2-loaded cells were excited at 340 and 380 nm and fluorescence at 510 nm was expressed as F340/F380 ratio, as an index of cytosolic Ca2+. Ca2+ stores were depleted with 1 µM ionomycin Ca in nominally Ca2+ free-tyrode solution, resulting in cytosolic Ca2+ peak.
  • Calcium increase
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vivo
    Verified Customer | Posted 10/02/2018
    Elicits calcium increase, used as a positive control at the end of calcium imaging experiments.

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