Ionomycin free acid
Chemical Name: (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Trihydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid
Biological ActivityIonomycin free acid is a calcium ionophore; more specific than A23187.
Calcium Salt also available.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Characterization of ionomycin as a calcium ionophore.
Liu and Hermann
Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria.
Kaufmann et al.
Ionomycin causes activation of p38 and p42/44 mitogen-activated protein kinases in human neutrophils.
Elzi et al.
Am.J.Physiol.Cell Physiol., 2001;281:C350
Citation for Ionomycin free acid
The citations listed below are publications that use Tocris products. Selected citations for Ionomycin free acid include:
1 Citation: Showing 1 - 1
Manipulation of Gut Microbiota Influences Immune Responses, Axon Preservation, and Motor Disability in a Model of Progressive Multiple Sclerosis.
Authors: Mestre Et al.
Front Immunol 2019;10:1374
No product specific FAQs exist for this product, however you mayView all Small Molecule FAQs
Reviews for Ionomycin free acid
Average Rating: 4.5 (Based on 2 Reviews)
Have you used Ionomycin free acid?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
HEK293 cells were loaded with 1 µM intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 30 min in dark, washed, and left to equilibrate for 15 min. Fura2-loaded cells were excited alternately at 340 and 380 nm and the fluorescence at 510 nm was expressed as 340/380 ratio to indicate intracellular Ca2+ levels. Application of 1 µM ionomycin in Ca2+-free tyrode solution released Ca2+ from intracellular stores, manifested as a transient peak in fluorescence ratio.
Ionomycin was used as a calcium ionophore to bring large amounts of extracellular Ca2+ into activated CD8+ T-cells. With high intracellular Ca2+, this led to increased activation of CD8+ T-cells as measured by IFNg production via intracellular flow cytometry.