Ionomycin free acid

Catalog # Availability Size / Price Qty
Ionomycin free acid | CAS No. 56092-81-0 | Calcium Signaling Agents
1 Image
Description: Calcium ionophore

Chemical Name: (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Trihydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid

Product Details
Citations (1)
Reviews (2)

Biological Activity

Ionomycin free acid is a calcium ionophore; more specific than A23187.

Calcium Salt also available.

Technical Data

Soluble to 10 mM in ethanol and to 10 mM in DMSO
Desiccate at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Other Product-Specific Information:

Product Datasheets

Or select another batch:
Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Molarity Calculator


*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Citation for Ionomycin free acid

The citations listed below are publications that use Tocris products. Selected citations for Ionomycin free acid include:

1 Citation: Showing 1 - 1


No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for Ionomycin free acid

Average Rating: 4.5 (Based on 2 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Ionomycin free acid?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Ionomycin releases Ca2+ from intracellular stores.
By Anonymous on 01/02/2020
Application: Species: Human

HEK293 cells were loaded with 1 µM intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 30 min in dark, washed, and left to equilibrate for 15 min. Fura2-loaded cells were excited alternately at 340 and 380 nm and the fluorescence at 510 nm was expressed as 340/380 ratio to indicate intracellular Ca2+ levels. Application of 1 µM ionomycin in Ca2+-free tyrode solution released Ca2+ from intracellular stores, manifested as a transient peak in fluorescence ratio.

Ionomycin increases flow of extracellular Ca2+ Into cells leading to increased T-cell activation by IFNg
By Li Zhu on 01/23/2019
Application: Species: Human

Ionomycin was used as a calcium ionophore to bring large amounts of extracellular Ca2+ into activated CD8+ T-cells. With high intracellular Ca2+, this led to increased activation of CD8+ T-cells as measured by IFNg production via intracellular flow cytometry.

Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.