Ionomycin free acid

Tocris Bioscience | Catalog # 2092

Calcium ionophore
Tocris Bioscience

Key Product Details

Description

Calcium ionophore
View all Calcium Signaling Agents »

Product Description

Ionomycin free acid is a calcium ionophore; more specific than A23187.

Calcium Salt also available.

Product Specifications for Ionomycin free acid

Molecular Weight

709.01

Formula

C41H72O9

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Trihydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid

CAS Number

56092-81-0

PubChem ID

6912226

InChI Key

PGHMRUGBZOYCAA-ADZNBVRBSA-N

SMILES

O=C(O)CC[C@@H](C)C[C@H](C)C[C@H](C)C(/C=C(O)/[C@H](C)C[C@@H](C/C=C/[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]1CC[C@]([C@]2([H])O[C@@]([C@H](O)C)(C)CC2)(C)O1)C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.09 10

Preparing Stock Solutions for Ionomycin free acid

The following data is based on the product molecular weight 709.01.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 14.10 mL 70.52 mL 141.04 mL
0.5 mM 2.82 mL 14.10 mL 28.21 mL
1 mM 1.41 mL 7.05 mL 14.10 mL
5 mM 0.28 mL 1.41 mL 2.82 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 8 publications citing the usage of this product.

Product Documents for Ionomycin free acid

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Ionomycin free acid

For research use only

Citations for Ionomycin free acid

Customer Reviews for Ionomycin free acid (2)

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  • Ionomycin releases Ca2+ from intracellular stores.
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 01/02/2020
    HEK293 cells were loaded with 1 µM intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 30 min in dark, washed, and left to equilibrate for 15 min. Fura2-loaded cells were excited alternately at 340 and 380 nm and the fluorescence at 510 nm was expressed as 340/380 ratio to indicate intracellular Ca2+ levels. Application of 1 µM ionomycin in Ca2+-free tyrode solution released Ca2+ from intracellular stores, manifested as a transient peak in fluorescence ratio.
    Ionomycin free acid 2092
  • Ionomycin increases flow of extracellular Ca2+ Into cells leading to increased T-cell activation by IFNg
    Name: Li Zhu
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Primary Cd8+ T-cells
    Verified Customer | Posted 01/23/2019
    Ionomycin was used as a calcium ionophore to bring large amounts of extracellular Ca2+ into activated CD8+ T-cells. With high intracellular Ca2+, this led to increased activation of CD8+ T-cells as measured by IFNg production via intracellular flow cytometry.
    Ionomycin free acid 2092

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