IRAK3 Antibody (1F6) - Azide and BSA Free

Novus Biologicals | Catalog # H00011213-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F6

Format

Azide and BSA Free
View all IRAK3 Primary Antibodies »

Product Specifications

Immunogen

IRAK3 (AAH57800, 497 a.a. ~ 596 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVPSQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDEYEQYKKE

Reactivity Notes

Human. Other species not tested.

Localization

Cell membrane

Specificity

IRAK3 - interleukin-1 receptor-associated kinase 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IRAK3 Antibody (1F6) - Azide and BSA Free

Western Blot: IRAK3 Antibody (1F6) [H00011213-M02]

Western Blot: IRAK3 Antibody (1F6) [H00011213-M02]

Western Blot: IRAK3 Antibody (1F6) [H00011213-M02] - Analysis of IRAK3 expression in transfected 293T cell line by IRAK3 monoclonal antibody (M02), clone 1F6.Lane 1: IRAK3 transfected lysate(67.8 KDa).Lane 2: Non-transfected lysate.
Western Blot: IRAK3 Antibody (1F6) [H00011213-M02]

Western Blot: IRAK3 Antibody (1F6) [H00011213-M02]

Western Blot: IRAK3 Antibody (1F6) [H00011213-M02] - IRAK3 monoclonal antibody (M02), clone 1F6 Western Blot analysis of IRAK3 expression in HeLa ( Cat # L013V1 ).
Western Blot: IRAK3 Antibody (1F6) [H00011213-M02]

Western Blot: IRAK3 Antibody (1F6) [H00011213-M02]

Western Blot: IRAK3 Antibody (1F6) [H00011213-M02] - Analysis of IRAK3 over-expressed 293 cell line, cotransfected with IRAK3 Validated Chimera RNAi ( Cat # H00011213-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK3 monoclonal antibody (M02) clone 1F6 (Cat # H00011213-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: IRAK3 Antibody (1F6) [H00011213-M02] - Detection limit for recombinant GST tagged IRAK3 is approximately 0.3ng/ml as a capture antibody.

Applications for IRAK3 Antibody (1F6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRAK3

This protein is a novel member in the IRAK/Pelle family. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and play a central role in IL-1R/TLR mediated inflammatory responses. Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.

Long Name

Interleukin-1 Receptor-Associated Kinase 3

Alternate Names

ASRT5, IRAK-M

Entrez Gene IDs

11213 (Human)

Gene Symbol

IRAK3

OMIM

604459 (Human)

UniProt

AAH57800 (Human)

Additional IRAK3 Products

Product Documents for IRAK3 Antibody (1F6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IRAK3 Antibody (1F6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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