Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5)

Novus Biologicals | Catalog # NBP3-19494

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-2G5 expressed in HEK293
View all Isocitrate Dehydrogenase 1/IDH1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Isocitrate Dehydrogenase 1/IDH1 (Uniprot # O75874)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5)

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5) [NBP3-19494]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5) [NBP3-19494]

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5) [NBP3-19494] - Western blot detection of Isocitrate Dehydrogenase 1/IDH1 in Rat Brain, C6, 3T3, Hela cell lysates using NBP3-19494 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5) [NBP3-19494]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5) [NBP3-19494]

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5) [NBP3-19494] - Immunohistochemistry of Isocitrate dehydrogenase in paraffin-embedded Human Cholangiocarcinoma using NBP3-19494 at dilution 1/20

Applications for Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5)

Application
Recommended Usage

Immunohistochemistry

1/20-1/100

Western Blot

1/1000-1/5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Isocitrate Dehydrogenase 1/IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Alternate Names

IDCD, IDH1, IDP, IDPC, PICD

Gene Symbol

IDH1

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Product Documents for Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5)

There are currently no reviews for this product. Be the first to review Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5) and earn rewards!

Have you used Isocitrate Dehydrogenase 1/IDH1 Antibody (S05-2G5)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies