Isopeptidase T/USP5 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-77286
![Immunohistochemistry: Isopeptidase T/USP5 Antibody [NB100-77286]](https://resources.rndsystems.com/images/products/Isopeptidase-T-USP5-Antibody-Immunohistochemistry-NB100-77286-img0006.jpg "Immunohistochemistry: Isopeptidase T/USP5 Antibody [NB100-77286]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 375 and 425 of human ubiquitin specific peptidase 5 (isopeptidase T) using the numbering given in entry CAA62690.1 (GeneID 8078).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Isopeptidase T/USP5 Antibody - BSA Free
Immunohistochemistry: Isopeptidase T/USP5 Antibody [NB100-77286]
Immunohistochemistry: Isopeptidase T/USP5 Antibody [NB100-77286] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP5/IsoT used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286]](https://resources.rndsystems.com/images/products/Isopeptidase-T-USP5-Antibody-Immunohistochemistry-Paraffin-NB100-77286-img0002.jpg "Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286]")
Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286]
Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-USP5 used at a dilution of 1:100. Detection: DAB staining using Immunohistochemistry Accessory Kit.![Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286]](https://resources.rndsystems.com/images/products/Isopeptidase-T-USP5-Antibody-Immunohistochemistry-Paraffin-NB100-77286-img0003.jpg "Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286]")
Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286]
Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NB100-77286] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-USP5used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit![Immunoprecipitation: Isopeptidase T/USP5 Antibody [NB100-77286]](https://resources.rndsystems.com/images/products/Isopeptidase-T-USP5-Antibody-Immunoprecipitation-NB100-77286-img0004.jpg "Immunoprecipitation: Isopeptidase T/USP5 Antibody [NB100-77286]")
Immunoprecipitation: Isopeptidase T/USP5 Antibody [NB100-77286]
Immunoprecipitation: Isopeptidase T/USP5 Antibody [NB100-77286] - Detection of Human USP5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP5 antibody Nb100-77286 used for IP at 3 ug/mg lysate. USP5 was also immunoprecipitated by rabbit anti-USP5 antibody NB100-77285, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for Isopeptidase T/USP5 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Isopeptidase T/USP5
Long Name
Ubiquitin Specific Protease 5
Alternate Names
IsoT, USP5
Entrez Gene IDs
8078 (Human)
Gene Symbol
USP5
UniProt
Additional Isopeptidase T/USP5 Products
Product Documents for Isopeptidase T/USP5 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Isopeptidase T/USP5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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