ITIH4 Antibody (CL1858) - BSA Free
Novus Biologicals | Catalog # NBP2-34492
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL1858
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q14624, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ITIH4 Antibody (CL1858) - BSA Free
![Western Blot: ITIH4 Antibody (CL1858) [NBP2-34492]](https://resources.rndsystems.com/images/products/ITIH4-Antibody-CL1858-Western-Blot-NBP2-34492-img0006.jpg "Western Blot: ITIH4 Antibody (CL1858) [NBP2-34492]")
Western Blot: ITIH4 Antibody (CL1858) [NBP2-34492]
Western Blot: ITIH4 Antibody (CL1858) [NBP2-34492] - Lane 1: Marker [kDa]. Lane 2: Human liver tissue lysate![Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]](https://resources.rndsystems.com/images/products/ITIH4-Antibody-CL1858-Immunohistochemistry-NBP2-34492-img0004.jpg "Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]")
Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]
Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492] - Staining of human cerebellum shows absence of immunoreactivity (negative control).![Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]](https://resources.rndsystems.com/images/products/ITIH4-Antibody-CL1858-Immunohistochemistry-NBP2-34492-img0001.jpg "Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]")
Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]
Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492] - Staining of human liver shows immunoreactivity in sinusoids.![Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]](https://resources.rndsystems.com/images/products/ITIH4-Antibody-CL1858-Immunohistochemistry-NBP2-34492-img0002.jpg "Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]")
Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]
Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492] - Staining of human fallopian tube shows strong immunoreactivity in blood vessels and in plasma.![Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]](https://resources.rndsystems.com/images/products/ITIH4-Antibody-CL1858-Immunohistochemistry-NBP2-34492-img0003.jpg "Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]")
Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492]
Immunohistochemistry: ITIH4 Antibody (CL1858) [NBP2-34492] - Staining of human kidney (renal cancer) shows strong positivity in plasma in blood vessels.Applications for ITIH4 Antibody (CL1858) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Frozen
Reported in scientific literature (PMID: 28724284)
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ITIH4
Long Name
Inter-alpha-Trypsin Inhibitor Heavy Chain H4
Alternate Names
gp120, H4P, IHRP, ITI-HC4, ITIHL1, PK120
Gene Symbol
ITIH4
UniProt
Additional ITIH4 Products
Product Documents for ITIH4 Antibody (CL1858) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ITIH4 Antibody (CL1858) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for ITIH4 Antibody (CL1858) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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